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2d gaussian curve fitting  (OriginLab corp)

 
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    Structured Review

    OriginLab corp 2d gaussian curve fitting
    A) Concentration-dependent fluorescence intensity from the extended fluorescent arm of eGFP-gp16. B, a) Enlarged single-molecule image showing individual immobilized eGFP-gp16 distributed over the observed area (70 × 70 μm). b) Point-spread-function fitting of the circled fluorescent spot. The pixel size is 160 nm. C) Photobleaching step analysis showing a) one- or b) two-step drop from the immobilized eGFP-gp16. Time 0 indicates the beginning of laser excitation. D) Distribution of fluorescence intensity of the spots that showed one (solid bars) or two (shaded bars) steps in photobleaching, respectively. Xc represents the peak value obtained from each superimposed <t>Gaussian</t> plot over the data points, with Xc = 5.86 (solidline) for those spots containing one gp16 molecule and Xc = 10.53 (dashed line) for those containing two gp16 molecules.
    2d Gaussian Curve Fitting, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2d gaussian curve fitting/product/OriginLab corp
    Average 90 stars, based on 1 article reviews
    2d gaussian curve fitting - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Engineering of the Fluorescent-Energy-Conversion Arm of Phi29 DNA Packaging Motor for Single-Molecule Studies"

    Article Title: Engineering of the Fluorescent-Energy-Conversion Arm of Phi29 DNA Packaging Motor for Single-Molecule Studies

    Journal:

    doi: 10.1002/smll.200900467

    A) Concentration-dependent fluorescence intensity from the extended fluorescent arm of eGFP-gp16. B, a) Enlarged single-molecule image showing individual immobilized eGFP-gp16 distributed over the observed area (70 × 70 μm). b) Point-spread-function fitting of the circled fluorescent spot. The pixel size is 160 nm. C) Photobleaching step analysis showing a) one- or b) two-step drop from the immobilized eGFP-gp16. Time 0 indicates the beginning of laser excitation. D) Distribution of fluorescence intensity of the spots that showed one (solid bars) or two (shaded bars) steps in photobleaching, respectively. Xc represents the peak value obtained from each superimposed Gaussian plot over the data points, with Xc = 5.86 (solidline) for those spots containing one gp16 molecule and Xc = 10.53 (dashed line) for those containing two gp16 molecules.
    Figure Legend Snippet: A) Concentration-dependent fluorescence intensity from the extended fluorescent arm of eGFP-gp16. B, a) Enlarged single-molecule image showing individual immobilized eGFP-gp16 distributed over the observed area (70 × 70 μm). b) Point-spread-function fitting of the circled fluorescent spot. The pixel size is 160 nm. C) Photobleaching step analysis showing a) one- or b) two-step drop from the immobilized eGFP-gp16. Time 0 indicates the beginning of laser excitation. D) Distribution of fluorescence intensity of the spots that showed one (solid bars) or two (shaded bars) steps in photobleaching, respectively. Xc represents the peak value obtained from each superimposed Gaussian plot over the data points, with Xc = 5.86 (solidline) for those spots containing one gp16 molecule and Xc = 10.53 (dashed line) for those containing two gp16 molecules.

    Techniques Used: Concentration Assay, Fluorescence



    Similar Products

    2d gaussian curve fitting  (OriginLab corp)
    90
    OriginLab corp 2d gaussian curve fitting
    A) Concentration-dependent fluorescence intensity from the extended fluorescent arm of eGFP-gp16. B, a) Enlarged single-molecule image showing individual immobilized eGFP-gp16 distributed over the observed area (70 × 70 μm). b) Point-spread-function fitting of the circled fluorescent spot. The pixel size is 160 nm. C) Photobleaching step analysis showing a) one- or b) two-step drop from the immobilized eGFP-gp16. Time 0 indicates the beginning of laser excitation. D) Distribution of fluorescence intensity of the spots that showed one (solid bars) or two (shaded bars) steps in photobleaching, respectively. Xc represents the peak value obtained from each superimposed <t>Gaussian</t> plot over the data points, with Xc = 5.86 (solidline) for those spots containing one gp16 molecule and Xc = 10.53 (dashed line) for those containing two gp16 molecules.
    2d Gaussian Curve Fitting, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2d gaussian curve fitting/product/OriginLab corp
    Average 90 stars, based on 1 article reviews
    2d gaussian curve fitting - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Concentration-dependent fluorescence intensity from the extended fluorescent arm of eGFP-gp16. B, a) Enlarged single-molecule image showing individual immobilized eGFP-gp16 distributed over the observed area (70 × 70 μm). b) Point-spread-function fitting of the circled fluorescent spot. The pixel size is 160 nm. C) Photobleaching step analysis showing a) one- or b) two-step drop from the immobilized eGFP-gp16. Time 0 indicates the beginning of laser excitation. D) Distribution of fluorescence intensity of the spots that showed one (solid bars) or two (shaded bars) steps in photobleaching, respectively. Xc represents the peak value obtained from each superimposed Gaussian plot over the data points, with Xc = 5.86 (solidline) for those spots containing one gp16 molecule and Xc = 10.53 (dashed line) for those containing two gp16 molecules.

    Journal:

    Article Title: Engineering of the Fluorescent-Energy-Conversion Arm of Phi29 DNA Packaging Motor for Single-Molecule Studies

    doi: 10.1002/smll.200900467

    Figure Lengend Snippet: A) Concentration-dependent fluorescence intensity from the extended fluorescent arm of eGFP-gp16. B, a) Enlarged single-molecule image showing individual immobilized eGFP-gp16 distributed over the observed area (70 × 70 μm). b) Point-spread-function fitting of the circled fluorescent spot. The pixel size is 160 nm. C) Photobleaching step analysis showing a) one- or b) two-step drop from the immobilized eGFP-gp16. Time 0 indicates the beginning of laser excitation. D) Distribution of fluorescence intensity of the spots that showed one (solid bars) or two (shaded bars) steps in photobleaching, respectively. Xc represents the peak value obtained from each superimposed Gaussian plot over the data points, with Xc = 5.86 (solidline) for those spots containing one gp16 molecule and Xc = 10.53 (dashed line) for those containing two gp16 molecules.

    Article Snippet: A 2D Gaussian curve fitting was then applied to the histograms by OriginPro 8 (OriginLab, USA).

    Techniques: Concentration Assay, Fluorescence